Influence of different culture media and light intensity on the growth and biomass productivity of a newly isolated Chlorella sp. UNM-IND1 from Waepella hot spring, South Sulawesi, Indonesia

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Indrayani Indrayani, Nur Zakiyah Ramadani, Nurul Mawaddah, Ernawati S. Kaseng, Andi Sukainah, Reski Praja Putra, Amiruddin Hambali, Ratnawaty Fadilah, Nurmila, Ardiansyah

2023 AACL Bioflux Vol. 16 Issue 3 Article Cited by 6 Quartile

Abstract

The Chlorella sp. UNM-IND1 is a newly isolated microalga species from Waepella hot spring in Sinjai Regency, South Sulawesi, Indonesia. As a newly isolated species, there is no information available regarding its optimum culture condition for high growth rate and biomass productivity. Therefore, this study aimed to analyze the growth and productivity of the alga under different culture media and light intensity. The alga was cultured under various culture media including F/2, Walne, Jaworski, and NPK+urea at salinity 0% NaCl and incubated at 25oC, the light intensity of 2500 lux with light and dark cycles of 12:12 hours. The best culture media was then used for the light-intensity study. The cultures were grown in NPK+urea media under different light intensities (2500, 3500, and 8000 lux) and incubated at 25oC with light and dark cycles of 12:12 hours. The alga was cultured for 2 weeks. The results showed that the highest growth (0.77 day-1) and biomass productivity (0.13 g L-1 day-1) was obtained when the alga was cultured in NPK+urea media. For the light intensity, the highest growth rate (0.94 day-1) was obtained at the highest light intensity (8000 lux). For the biomass productivity, both light intensity of 3500 and 8000 lux have equal biomass productivity of about 0.311 and 0.310 g L-1 day-1, respectively. This study indicated that the microalga Chlorella sp. UNM-IND1 prefers NPK+urea medium and high light intensity for higher growth rate and biomass productivity. © 2023, BIOFLUX SRL. All rights reserved.

Affiliations

Study Program of Agricultural Technology Education, Faculty of Engineering, Universitas Negeri Makassar, South Sulawesi, Makassar, 90224, Indonesia; Department of Aquaculture, Pangkep State Polytechnic of Agriculture, South Sulawesi, Pangkep, Indonesia