Alimuddin Ali, Muharram Passitta, Herlina Rante, Elly Wahyudin, Nana Juniarti Natsir Djide, Roland Jansen Politan, Elyza Aiman Nur, Satoru Shigeno, Satoshi Ohte, Keisuke Kobayashi, Kanji Hosoda, Hiroshi Tomoda, Taichi Ohshiro
This study investigates the antibiotic production potential of endophytic actinomycetes isolated from Poikilospermum suaveolens plants. Samples were collected from various parts of the plant in karst ecosystems. Endophytic actinomycetes were cultured and screened for antimicrobial activity using agar diffusion and liquid microdilution methods. One of the 11 isolated strains exhibited significant antibiotic activity against clinically relevant organisms identified as belonging to the Streptomyces genus, specifically Streptomyces mirabilis strain NRRL ISP-5169. Submerged fermentation of this strain over 9 days showed strong antagonistic effects against Gram-positive bacteria. Spectroscopic analyses confirmed the presence of actinomycin D as the bioactive compound. This study provides on the previously unexplored potential of endophytic actinomycetes from P. suaveolens as a promising resource for antibiotic discovery. © 2024, Society for Indonesian Biodiversity. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Jl. Daeng Tata, South Sulawesi, Makassar, 90222, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Jl. Daeng Tata, South Sulawesi, Makassar, 90222, Indonesia; Faculty of Pharmacy, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, South Sulawesi, Makassar, 90245, Indonesia; Department of Microbial Chemistry, Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, 108-8641, Japan; Medicinal Research Laboratories, Graduate School of Pharmaceutical Sciences Kitasato University, Tokyo, 108-8641, Japan; Laboratory of Drug Discovery, Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, 108-8641, Japan
