Amiruddin, Lili Darlian, Sriyana Herman, Mayang Sari, Rusli
Background and Objective: Infertility is still a phenomenon in the community, so consuming Moringa leaves (Moringa oleifera Lam.) is expected to increase fertility. This study aimed to determine the effect of Moringa leaf extract (Moringa oleifera Lam.) on the diameter of the primary and secondary follicles in female mice (Mus musculus). Materials and Methods: This study was an experiment using a Completely Randomized Design (CRD). The population of this study was 45 mice and samples were obtained by a simple random sampling technique from as many as 24 mice with the following criteria: Weight 20-25 g, 2-3 months old, female and in good health. Data analysis was performed through the ANOVA Test with a confidence level of α = 0.05 and further tested for the least significant difference (LSD). Results: Moringa leaf extract significantly positively affects the diameter of primary and secondary follicles in female mice (p<0.05). The average primary follicle diameter was P0 (92.65 µm), P1 (124.92 µm), P2 (150.72 µm), P3 (175.68 µm) and the average secondary follicle diameter was control (157.17 µm), P1 (171.33 µm), P2 (204.57 µm), P3 (211.11 µm). Giving Moringa leaf extract (Moringa oleifera Lam.) significantly increases the diameter of mice’s primary and secondary follicles due to the presence of vitamin E in Moringa leaf extract (Moringa oleifera Lam.). Conclusion: This can stimulate granulosa cells to secrete the hormone estrogen, causing an increase in the diameter of the primary and secondary follicles. © 2023 Amiruddin et al.
Department of Biology Education, Halu Oleo University, Sulawesi Tenggara, Kota Kendari, 93232, Indonesia; Department of Reproductive Health, University of Megarezky Makassar, Sulawesi Selatan, Kota Makassar, 90234, Indonesia; Department of Nutrition, Makassar State University, Sulawesi Selatan, Kota Makassar, 90222, Indonesia
