Anita, Hasnah Natsir, Paulina Taba, Ahmad Ahyar, Nunuk Hariani Soekamto, Nur Umriani Permatasari, Wahyudin Rauf, Sarlan, Nasrum Massi, Sudding, Andi Fatmawati
Marine ecosystems are invaluable sources of biodiversity and unique chemical compounds, especially for the discovery of new natural products, including enzymes. Marine sediment bacteria from the Losari Coastal, Makassar City, Indonesia, show great potential for biotechnological applications due to their ability to produce enzymes with extraordinary properties such as salt tolerance, heat stability, and adaptability to extreme conditions. This study investigated the enzymatic capabilities of marine sediment bacteria isolated from the Losari Coastal, Makassar City, Indonesia, focusing on 3 enzymes: Amylase, lipase, and protease. Bacterial samples were collected from 4 coastal locations, and a comprehensive analysis of their morphological, physiological, and molecular characteristics was performed, combining 16S rRNA sequencing and protein modeling using SWISS-MODEL. This study yielded 15 bacterial isolates characterized by a gram-negative, rod-shaped morphology and distinctive, round, yellow colonies with serrated edges. Among these, 10 produced amylase, 9 produced lipases, and 10 showed protease activity; however, 2 isolates stood out because they could produce all three enzymes: amylase, lipase, and protease. The MB-SL 5 isolate showed 97.82% similarity to Shewanella algae strain DW01, and the MB-SL 6 isolate had 90.53% similarity to Vibrio alginolyticus NBRC 15630 strain, with predicted protein structure code A0A3M5GRJ4_1 A. These findings highlight the significant biotechnological potential of marine sediment bacteria from the Losari Coastal producing hydrolytic enzymes for industrial applications, which opens new avenues for the development of new biocatalysts. © 2025, Walailak University. All rights reserved.
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, South Sulawesi, 90245, Indonesia; Departement of Microbiology, Faculty of Medicine, Hasanuddin University, South Sulawesi, 90245, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, South Sulawesi, 90222, Indonesia; Medical Laboratory Technology, Polytechnic of Muhammadiyah Makassar, South Sulawesi, 90132, Indonesia
