Maryono, Netti Herawati, Meuthia Aulia Farhani Gaffar, Sartini, Aliyah, Elly Wahyudin
Rice Bran (Oryza sativa L. cv Cilliwung) is a by-product of the rice milling process, consisting of an outer layer of rice grains with the whole seed institute. Rice bran oil is a result of rice bran extraction. Rice bran oil contains antioxidant compounds, one of which is oryzanol, so this study aims to determine the levels of or-oryzanol in rice bran. The stages of the research included sample preparation, extraction using Microwave-Assisted Extraction (MAE), followed by Vacuum Liquid Column Chromatography (VLCC) was extracted using the MAE method produced 30 mL of black-brown rice bran oil extract. A total of 8 grams of rice bran extract was obtained at VLCC using eluent (n-hexane: ethyl acetate) with ratios 9:1, 7:3, and 1:1, respectively. Then the five factions obtained were characterized using HPLC, and GC/MS identified the mass of the γ-oryzanol compound. © 2021 Phcogj.Com.
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Makassar State University, South Sulawesi, Makassar, Indonesia; Faculty of Pharmacy, Hasanuddin University, South Sulawesi, Makassar, Indonesia
