Indrayani Indrayani, Haslianti Haslianti, Asmariani Asmariani, Wellem H. Muskita, Ardiansyah, Herlan Hidayat
Salinity affects growth and biochemical composition of microalgae and the ability of microalgae to tolerate wide range of salinity is one of the important criteria for successful mass cultivation in outdoor open pond systems for any commercial application. The aim of this study was to determine the growth and lipid production of the newly isolated marine microalga Nannochloropsis sp. UHO3 at increasing salinity. The strain was isolated from a coastal area in Kendari, Southeast Sulawesi, Indonesia in June 2017. The strain was cultured in 500 mL Schott bottles containing 300 mL f/2 medium at increasing salinities from 2 to 7% NaCI, light intensity of about 100 |jmol photon m-2 s-1, 12:12 hours light and dark cycles at ambient room temperatures. The highest specific growth rate (0.779±0.02 d-1) was achieved at 3% salinity and the lowest (0.455±0.02d-1) was obtained at 7% salinity. The cultures grown at 3% salinity had the highest lipid content and lipid productivity (22.06±2.92% Ash-Free Dry Weight (AFDW) and 0.161±0.009g L-1 d-1, respectively). This study suggests that the alga has a wide salinity tolerance (2-7% NaCl) and produce high lipid at 3-4% salinity. Hence, the species is potential for outdoor mass cultivation in saline-hypersaline media for biodiesel feedstock due to its high growth rate and lipid productivity. © 2021, BIOFLUX SRL. All rights reserved.
Study Programme of Agricultural Technology Education, Faculty of Engineering, Makassar State University, Parangtambung Campus, Makassar, South Sulawesi, 90224, Indonesia; Department of Fisheries Products Technology, Faculty of Fisheries and Marine Sciences, University of Halu Oleo, Kendari, Southeast Sulawesi, 93232, Indonesia; Fisheries Laboratory, Faculty of Fisheries and Marine Sciences, University of Halu Oleo, Kendari, Southeast Sulawesi, 93232, Indonesia; Department of Aquaculture, Faculty of Fisheries and Marine Sciences, University of Halu Oleo, Kendari, Southeast Sulawesi, 93232, Indonesia; Aquaculture Department, Agricultural Polytechnic State of Pangkep, Makassar-ParePare Km. 83, South Sulawesi, Indonesia; Department of Environmental Science, Faculty of Environmental Science and Forestry, University of Halu Oleo, Kendari, Southeast Sulawesi, 93232, Indonesia
