Ardiansyah, Amrullah, Dahlia, Indrayani, Muhariyadi Atmomarsono, Firmansyah Bin A. Jabbar
Shrimp production is affected by disease attacks caused by various types of pathogenic Vibrio species. Mucus secreted in the epidermis of the fish skin acts as the first line of defence between fish and pathogens in their living medium. The purpose of this study was to evaluate the antimicrobial potential of Nile tilapia (Oreochromis niloticus) epidermal mucus against pathogenic bacteria in white shrimp (Litopenaeus vannamei Boone). Crude, acid, and aqueous mucin extracts of the epidermis were prepared, and their antibacterial activity was tested by disc diffusion method against three bacterial pathogens of vannamei shrimp; Vibrio harveyi, V. parahaemolyticus, and V. alginolyticus. The antibacterial activity was measured in the zone of inhibition in mm and compared with the antibiotic Streptomycin as a positive control, distilled water, and acetic acid as negative controls. Of the 27 tests performed (three types of epidermal mucin extract against three different bacterial strains with three repetitions), 15 tests showed antibacterial activity. Acid extracts showed inhibitory potential against pathogenic bacterium V. harveyi, crude and acidic extracts were able to inhibit V. parahaemolyticus, while V. alginolyticus could be inhibited by the three types of epidermal mucin extracts. O. niloticus acid extract showed inhibitory activity for all types of test pathogens; V. harveyi, V. parahaemolyticus, and V. alginolyticus. The acidic extract of O. niloticus significantly affected V. harveyi, V. parahaemolyticus, and V. alginolyticus at a minimum concentration of 20 µL mL-1, but the aqueous extract of O. niloticus required 60 µL mL-1 of protein to inhibit V. harveyi. O. niloticus epidermal mucin can be a natural product, which can help overcome the problem of antibiotic resistance of many pathogenic bacteria in shrimp culture. © 2021, BIOFLUX SRL. All rights reserved.
Pangkep State Polytechnic of Agriculture, South Sulawesi, Indonesia; Makassar State University, South Sulawesi, Makassar, Indonesia; Research Institute for Brackishwater Aquaculture and Fisheries Extension, Soth Sulawesi, Maros, Indonesia; University of West Sulawesi, West Sulawesi, Indonesia
