Effect of Extraction and Fractionation on Antioxidant Activity of Extract and Fraction Crescentia Cujete L Leaves

Open

Hartati, Abd Muis, Narhaeda, Hasmida Mohd Nasir, Nur Salsabila Md Norodin

2019 IOP Conference Series: Materials Science and Engineering Vol. 551 Issue 1 Conference paper Cited by 0 Quartile

Abstract

Antioxidant compunds like phenols and flavonoids scavenge free radicals and thus inhibit the oxidative mechanisms that lead to control degenerative and other diseases. The aim of this study was to investigate the antioxidant activity in extracts and fractions of Crescentia cujete leaves. 1,1-difenil-2-pikrilhidrazil (DPPH) assay was performed to measure the free-radical scavenging activity of extracts. The results of the study show that leaves of C. cujete possesses significant free radical scavenging properties. All the ethanol extract, etyl acetat extract and fractions exhibited antioxidant activities, however, ethanol fractions of leaves showing the highest antioxidant activity based on the results of DPPH tests. © 2019 Published under licence by IOP Publishing Ltd.

Affiliations

Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Indonesia; Centre of Lipids Engineering and Applied Research (CLEAR), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, Johor, 81310 UTM, Johor Bahru, Malaysia; School of Chemical and Energy Engineering, Faculty Engineering, Universiti Teknologi Malaysia, Johor, 81310, Malaysia