Ismail, Reny Syahruni, Alimuddin Ali, A. LilisSugiana, Sujud ZainurRosyid
The purpose of this study is to isolate, identify and evaluate of the antibacterial activities against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 83125 of endophytic fungi isolated from Acacia rugata (Lam.) fawc. Rendle. Three morphologically distinct endophytic fungi were isolated and identified by characterization macroscopically and microscopically, based on the results of the antagonistic test obtaining one active isolate. The recovered one isolate was further cultivated in Maltosa Yeast Broth; extracellular bioactive metabolites from the cultivating process had been extracted using ethyl acetate and its antibacterial activity was tested by disc agar diffusion method. Three isolates of endophytic fungi have antibacterial activity. The LC1 fungal extract showed a strong growth inhibitory effect against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 83125 with values of 10.4 mm and 11.5 mm respectively that were characterized having around configuration with jagged edge, the edge is wooly and crateri form elevation. It was identified as Arthrocristula sp. The presence of alkaloid and tannin compounds was identified in LC1 isolates. This study indicated that three fungi isolated from A. rugata leaves can produce compounds that are able to inhibit the growth of bacteria. LC1 isolate is identified as Arthrocristula sp. that contains alkaloids and tannins. © 2018 World Research Association. All Rights Reserved.
Sekolah Tinggi Ilmu Farmasi Makassar, Perintis Kemerdekaan Street Km 13.7, Daya, South Sulawesi, 90242, Indonesia; Akademi Farmasi Kebangsaan Makassar, Perintis Kemerdekaan Street Km 13.7, Daya, South Sulawesi, 90242, Indonesia; Department of Biology, Mathematic and Natural Sciences Faculty, Universitas Negeri Makassar, Mallengkeri Street No.44, Parang Tambung, Tamalate, Makassar, South Sulawesi, 90224, Indonesia
