Characterization and in vitro antifungal assay against Fusarium oxysporum f.sp. passiflora of endophytic actinomycetes from purple passion fruit plants of South Sulawesi, Indonesia

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Alimuddin Ali, Muhammad Junda, Hilda Karim, Sri Nuryani

2017 Asian Journal of Microbiology, Biotechnology and Environmental Sciences Vol. 19 Issue 2 Article Cited by 2

Abstract

Endophytic actinomycetes from purple passion fruit were screened and evaluated for their antifungal activity against pathogenic fungi. A total of 32 isolates were obtained from people passion garden in Malino distric, South Sulawesi province, Indonesia. Morphological and chemotaxonomical analysis indicated that twenty-two isolates belonged to the streptomyces like-strain. This genus was the most dominant among the isolates (68.75%) of plant organs (roots, leaves and stem barks) of these showed inhibitory activity against Fusarium oxysporum f.sp. passiflora. Actinomycetes were most commonly recovered from leaves (50% of all isolates), followed by roots (43.75%) and stems (6.25%). Based on the phenotypic properties and phylogenetic and analysis of 16S rRNA gene, the selected strain EML-D1A5 was assigned to Streptomyces durhamensis strain CSSP538 (97% similarity). © Global Science Publications.

Affiliations

Laboratory of Microbiology, Departement of Biology, Faculty of Mathematic and Natural Sciences, Universitas Negeri Makassar, South Selatan, 90233, Indonesia; Graduate School of Biology Departement, FMIPA, Universitas Negeri Makassar, Indonesia