Sitti Faika Ahsan, Surahmat HAMID Surahmat, Netti Herawati, Halimah Husain Halimah
Moringa (Moringa oleifera) is a plant that contains active ingredients as a result of secondary metabolism that can be utilized as traditional medicine. Moringa oleifera (MO) can overcome various diseases, such as diabetes, gout, and cancer. This study aimed to evaluate the influence of methanol concentration and acid addition on the anti-cholesterol activity and secondary metabolite profile of Moringa oleifera root extract (EMr), as well as to determine the content of secondary metabolite compounds in the EMr of MO roots. The research methods included maceration extraction, phytochemical screening, yield test, LCMS/MS analysis, Escherichia coli antibacterial test, and anti-cholesterol test on mice. Data were analyzed with SPSS (version 24) using the ANOVA test followed by the Tukey test. The results showed that the concentration of methanol solvent with acid addition (MO4) significantly retarded Escherichia coli growth at 6% concentration with a Minimum Inhibitory Concentration (MIC) of 19 mm (p<0.05), and demonstrated greater efficacy compared to MO1, MO2, MO3, MO5, and MO6. Phytochemical screening indicated the presence of flavonoid, alkaloid, and terpenoid compounds, and the results of LCMS/MS analysis identified 21 verified compounds. The results also showed that MO root EM has anti-cholesterol activity at 400 mg doses (p<0.05). © 2025 Visagaa Publishing House.
Department of Chemistry, Universitas Negeri Makassar, Jl. Daeng Tata Raya, South Sulawesi, Makassar, 90224, Indonesia; Department of Biomedical Science, Hasanuddin University, Jl. Perintis Kemerdekaan KM.10, South Sulawesi, Makassar, 90245, Indonesia
