Andi Besse Khaerunisa, Indah Raya, Hasnah Natsir, Nunuk Hariani Soekamto, Abdul Wahid Wahab, Rugaiyah Arfah, Rizal Irfandi, Eka Pratiwi, Wahyudin Rauf, Andi Adillah Nur Syafirah
This study aims to synthesize, characterize, and evaluate the anticancer potential of a manganese(II) cysteine-alanine dithiocarbamate complex to overcome challenges posed by drug resistance in breast cancer treatment. The Mn(II) cysteine-alanine dithiocarbamate complex was synthesized using manganese chloride, alanine, cysteine, and carbon disulfide under controlled conditions. The synthesized complex exhibited strong thermal stability (melting point: 200 – 218 °C) and weak electrolyte behavior (conductivity: 1.5 mS/cm at 28 °C). Characterization techniques included UV-Vis (absorption peaks at 253, 274, 284, 294, and 305 nm), FTIR (notable bands at 3338.78, 2918.30, 1597.06 and 689.30 cm⁻¹), XRD (sharp peaks at 28°, 32°, 43°, 60°, lattice parameters a = 3.40 Å, b = 2.48 Å), and SEM-EDS (porous morphology with dominant Mn and S content). Molecular docking revealed strong interactions with estrogen receptor alpha (ERα) through hydrogen bonds, van der Waals forces, and salt bridges, with a binding energy of −59.93 kJ/mol. Cytotoxicity was evaluated on MCF-7 cells using the MTS assay, revealing a dose-dependent inhibition with an IC50 value of 61.66 µg/mL. The Mn(II) cysteine-alanine dithiocarbamate complex exhibits promising structural stability, receptor binding affinity, and cytotoxic potential against MCF-7 breast cancer cells, highlighting its potential as an effective and selective anticancer agent. © 2025, Walailak University. All rights reserved.
Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University, Makassar, 90245, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Makassar, Makassar, 90244, Indonesia; Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, 90245, Indonesia
