Subaer Subaer, Hartati Hartati, Imam Ramadhan, Harlyenda Ismayanti, Teguh Wibawa, Agnes Sriyanti
AuNPs (gold nanoparticles) are a type of nanomaterial with high biocompatibility. This research aims to use the Turkevich method to produce AuNPs utilizing HAuCl4.3H2O as a precursor and Na3C6H5O7 as a reduction agent. UV-Vis Spectrophotometer (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM) characterizations of the resulting AuNPs revealed excellent band of Surface Plasmon Resonance (SPR) of about 542 nm, high crystallinity, variable shape, and a size range of 20-40 nm. This feature indicates that the AuNPs produced can be used in biomedical applications. Chitosan is an excellent antibacterial biomaterial. Shrimp shells were used to synthesize chitosan nanoparticles (CsNPs) in three stages: deproteination, demineralization, and deacetylation. UV-Vis, FTIR, and XRD techniques were used to characterize the resultant chitosan, and it was discovered to be of good quality. The antibacterial properties of chitosan nanoparticles (CsNPs) conjugated with AuNPs-Cs (AuNPs-Cs) were investigated using the agar diffusion method. Conjugation of AuNPs-CsNPs was found to diminish the growth of Escherichia coli bacteria effectively but not Staphylococcus aureus or Enterococcus faecalis bacteria. The findings of this study will open a better understanding of AuNPs-CsNPs conjugate as one of the biomedical materials because of their remarkable qualities. © 2024 Author(s).
Materials Physics Lab., Physics Department, Universitas Negeri Makassar, Makassar, Indonesia; Biology Department, Universitas Negeri Makassar, Makassar, Indonesia
